Laboratory of Genome Editing
The Laboratory of Genome Editing was organized on January 1, 2019 as a result of the separation of the genome editing group from the Laboratory of Mutagenesis.
- Sergeeva Vasilina AleksandrovnaSenior Researcher
- Lavrov Alexander VyacheslavovichLeading Researcher
- Panchuk Irina OlegovnaResearcher
- Levchenko Olga AlexandrovnaResearcher
- Zaynitdinova Milyausha IrshatovnaResearcher
- Anuchina Arina ArturovnaResearcher
- Slesarenko Yana SergeevnaResearcher
- Kondratyeva Ekaterina VladimirovnaResearcher
- Belova Lyubava LeonidovnaResearcher
- Demchenko Anna GasimovnaJunior Researcher
- Ivanova Alisa VladimirovnaJunior Researcher
- Vasilyeva Elena VladimirovnaResearch Assistant
About the Laboratory
The laboratory's efforts are focused on the topic of genome editing, including fundamental research for increasing the level of homologous recombination in CRISPR/Cas9 method, as well as applied research to develop treatment of hereditary monogenic diseases.
The following works are carried out in the laboratory:
- Editing the most frequent mutation F508del in CFTR gene for the treatment of cystic fibrosis.
- Editing mutations in the DES gene for the treatment of hereditary cardiomyopathies.
- Single nucleotide editing of mutations in the KERA gene for the treatment of autosomal recessive Cornea plana disease.
Cystic fibrosis is one of the most common monogenic diseases. The electrolyte composition in the extracellular medium is disturbed due to mutations in the gene of the channel protein for chlorine ions (CFTR), so results to disruption of many organs. Pulmonary symptoms are dominant clinical features. Lungs’ pathology determines the prognosis and survival of the patients. Etiology-based therapy of this disease does not exist. Developed technologies of highly efficient target genome editing allow us to hope that such therapy will be worked out in the near future for many hereditary diseases. Using the technology of targeted nucleases CRISPR/Cas9, we are developing a method for correcting the most frequent mutation p.F508del in cystic fibrosis.
- Voldgorn YI, Adilgereeva EP, Nekrasov ED, Lavrov AV. Cultivation and Differentiation Change Nuclear Localization of Chromosome Centromeres in Human Mesenchymal Stem Cells. PLoS ONE, 2015, 10(3): e0118350. doi: 10.1371/journal.pone.0118350 http://dx.plos.org/10.1371/journal.pone.0118350
- Lavrov A.V., Chelysheva E.Y., Smirnikhina S.A., Shukhov O.A., Turkina A.G., Adilgereeva E.P., Kutsev S.I. Frequent variations in cancer-related genes may play prognostic role in treatment of patients with chronic myeloid leukemia. BMC Genetics, 2016, 17(Suppl 1):14, DOI: 10.1186/s12863-015-0308-7 http://bmcgenet.biomedcentral.com/articles/10.1186/s12863-015-0308-7
- Smirnikhina S.A., Lavrov A.V., Chelysheva E.Yu., Adilgereeva E.P., Shukhov O.A., Turkina A.G., Kutsev S.I. Whole-exome sequencing reveals potential molecular predictors of relapse after discontinuation of the targeted therapy in CML. Leukemia and Lymphoma, 2016, DOI: 10.3109/10428194.2015.1132420 http://www.tandfonline.com/doi/full/10.3109/10428194.2015.1132420
- Lavrov AV, Ustaeva OA, Adilgereeva EP, Smirnikhina SA, Chelysheva EY, Shukhov OA, Shatokhin YV, Mordanov SV, Turkina AG, Kutsev SI. Copy number variation analysis in cytochromes and glutathione S-transferases may predict efficacy of tyrosine kinase inhibitors in chronic myeloid leukemia. PLoS ONE, 2017 12(9):e0182901. https://doi.org/10.1371/journal.pone.0182901. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0182901
- Smirnikhina SA, Chelysheva EY, Lavrov AV, Kochergin-Nikitsky KS, Mozgovoy IV, Adilgereeva EP, Shukhov OA, Petrova AN, Bykova AV, Abdullaev AO, Turkina AG, Kutsev SI. Genetic markers of stable molecular remission in chronic myeloid leukemia after targeted therapy discontinuation. Leuk Lymphoma. 2018 Feb 9:1-4. doi: 10.1080/10428194.2018.1434880. https://www.tandfonline.com/doi/abs/10.1080/10428194.2018.1434880?journalCode=ilal20
- Yakushina V.D., Lerner L.V., Lavrov A.V. Gene fusions in thyroid cancer. Thyroid. February 2018, 28(2): 158-167. https://doi.org/10.1089/thy.2017.0318
- Smirnikhina SA, Anuchina AA., Lavrov AV. Ways of improving precise knock-in by genome editing technologies. Human Genetics, 2018, Hum Genet. 2019 Jan;138(1):1-19. doi: 10.1007/s00439-018-1953-5. https://link.springer.com/article/10.1007%2Fs00439-018-1953-5
- Lavrov AV, Chelysheva EYu, Adilgereeva EP, Shukhov OA, Smirnikhina SA, Kochergin-Nikitsky KS, Yakushina VD, Tsaur GA, Mordanov SV, Turkina AG, Kutsev SI. Exome, transcriptome and miRNA analysis don’t reveal any molecular markers of TKI efficacy in primary CML patients. BMC Medical Genomics, 2019, doi: 10.1186/s12920-019-0481-z. https://bmcmedgenomics.biomedcentral.com/articles/10.1186/s12920-019-0481-z
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