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RCMG Has Developed Unique Protocols for Handling Human Pulmonary and Bronchial Organoids

The article on obtaining and using both types of organoids was published in the journal «International Journal of Molecular Sciences» (Q1, impact factor 6,208).

The staff of the genome editing laboratory with the participation of the stem cell genetics laboratory at RCMG developed unique protocols for the production of bronchial and pulmonary organoids from induced pluripotent human stem cells. Several organoids have been obtained from healthy donors and cystic fibrosis patients.

The published article contains step-by-step protocols for obtaining pulmonary and bronchial organoids. The genome editing laboratory of the Research Centre for Medical Genetics is the only one in Russia who possesses these techniques. For the first time in the world, RCMG staff showed that pulmonary organoids can be used in functional tests to determine the conductivity of a CFTR channel that is disrupted by cystic fibrosis. Lung organoids models can be used in the future for personalized drug screening, intercellular interaction research, lung pathogenesis studies, and the development of new treatments, including genomic editing.

Follow the link to read the full text of the article: https://www.mdpi.com/1422-0067/24/7/6293

Demchenko A, Kondratyeva E, Tabakov V, Efremova A, Salikhova D, Bukharova T, Goldshtein D, Balyasin M, Bulatenko N, Amelina E, Lavrov A, Smirnikhina S. Airway and Lung Organoids from Human-Induced Pluripotent Stem Cells Can Be Used to Assess CFTR Conductance. Int. J. Mol. Sci. 2023, 24, 6293. https://doi.org/10.3390/ijms24076293