Research Centre for Medical Genetics
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RCMG Determined the Importance of Several Factors Involved in DNA Repair in Genomic Editing to Correct Mutations and Improve the Efficiency of the Process

The report was published in the “International Journal of Molecular Sciences” (Q1, impact-factor 6,208).

Employees of the genome editing laboratory of the Research Centre for Medical Genetics managed to improve the exact correction of mutation in the model system by 10.2 times when inhibiting a number of factors involved in DNA repair.

The study sought how to improve homologous reparations when using CRISPR-Cas9 by influencing three key factors that regulate the balance between homologous recombination and nonhomologous end joints: MAD2L2, SCAI and ligase IV.

The results showed that inhibition of MAD2L2 and the addition of ligase inhibitor IV SCR7 significantly increased the effectiveness of mutation correction in the EGFP gene in the HEK293T cell line.

This study shows for the first time in the world that knockdown MAD2L2 during CRISPR-mediated gene editing in HEK293T cells can increase the exact correction to 10.2 times. Thus, the discovery of new DNA recovery factors may become a further strategy for the successful use of CRISPR-Cas systems.

Follow the link to read the full text of the article:

Anuchina AA, Zaynitdinova MI, Demchenko AG, Evtushenko NA, Lavrov AV, Smirnikhina SA. Bridging Gaps in HDR Improvement: The Role of MAD2L2, SCAI, and SCR7. Int. J.Mol. Sci. 2023, 24, 6704.