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Article by FSBI RCMG researchers in Oxidative Medicine and Cellular Longevity journal

Oxidative Medicine and Cellular Longevity journal published an article (impact-factor 4,936, Q1) by the FSBI RCMG Molecular Biology Laboratory team (headed by Kostyuk S.V., Doctor of biological science):

“Increased transfection of the easily oxidizable GC-rich DNA fragments into the MCF7 breast cancer cell”.

Authors: Svetlana Kostyuk, Nadezhda Mordkovich, Natalya Okorokova, Vladimir Veiko, Elena Malinovskaya, Elizaveta Ershova, Marina Konkova, Ekaterina Savinova, Maria Borzikova, Tatiana Muzaffarova, Nataly Veiko, Serguey Kutsev.

Easily oxidizable GC-rich DNA (GC-DNA) fragments accumulate in the cell-free DNA (cfDNA) of patients with various diseases. The human oxidized DNA penetrates the MCF7 breast cancer cells and significantly changes their physiology. This assumption was proved by experiments when MCF7 cells were cultured in the presence of pEGFP and pEGFP-rDNA plasmids. Insertions of the rDNA significantly increase the GC-DNA oxidation degree as well as the rate of plasmid transfection into the cells and the EGFP fluorescent protein expression level.

Thus, the oxidizable GC-DNA fragments can readily penetrate the cancer cells and be expressed.

This feature is important for understanding cancer cell survival during therapy. The cfDNA can carry mutant genes from the tumor genome that trigger carcinogenesis in healthy cells and lead to metastasis. It is not excluded that cfDNA may become a target for the antitumor therapy.

Oxidative Medicine and Cellular Longevity
Volume 2019, Article ID 2348165, 15 pages