Analysis of candidate genes expected to be essential for melanoma surviving

Selective targeting of the genes vital for tumor cells survival may treat Cancers. A number of attempts have led to discovery of several genes essential for surviving of tumor cells of different types. This work analyzed genes that were previously predicted to be essential for melanoma surviving. For this purpose a siRNA-mediated knockdown of the genes was made, and namely, UNC45A, STK11IP, RHPN2 and ZNFX1, in melanoma cell line A375. Then, it was evaluated how the reduced expression of the genes under study affected cell viability, cell proliferation, and migration ability.

It was shown that knockdown of genes necessary for melanoma survival did not result in statistically significant changes in cell viability. On the other hand, knockdown of each of the target genes was shown to accelerate the speed of cell migrating.

Krivosheeva IA, Filatova AY, Moshkovskii SA, Baranova AV, Skoblov MY. Analysis of candidate genes expected to be essential for melanoma surviving. Cancer Cell Int. 2020 Oct 7;20:488. doi: 10.1186/s12935-020-01584-2. eCollection 2020. (IF 3.439, Q2).

https://cancerci.biomedcentral.com/articles/10.1186/s12935-020-01584-2